Cross-Kingdom Amplification Using Bacteria -Specific Primers: Complications for Studies of Coral Microbial Ecology
نویسندگان
چکیده
منابع مشابه
Cross-kingdom amplification using bacteria-specific primers: complications for studies of coral microbial ecology.
PCR amplification of pure bacterial DNA is vital to the study of bacterial interactions with corals. Commonly used Bacteria-specific primers 8F and 27F paired with the universal primer 1492R amplify both eukaryotic and prokaryotic rRNA genes. An alternative primer set, 63F/1542R, is suggested to resolve this problem.
متن کاملCross - Kingdom Amplification Using Bacterial - Specific Primers : Complications for 1 Coral Microbial Ecology
9 PCR amplification of pure bacterial DNA is vital to the study of bacterial interactions 10 with corals. Commonly used bacterial-specific primers 8F and 27F paired with universal 11 primer 1492R amplify both eukaryotic and prokaryotic rDNA. An alternative primer set, 12 63F/1542R, is suggested to resolve this problem. 13
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ژورنال
عنوان ژورنال: Applied and Environmental Microbiology
سال: 2008
ISSN: 0099-2240,1098-5336
DOI: 10.1128/aem.01303-08